Bi-Enzymatic Conductometric Biosensor for Detection of Heavy Metal Ions and Pesticides in Water Samples Based on Enzymatic Inhibition in Arthrospira platensis

Biocapteur bimézymétrique conductimétrique pour la détection d'ions des métaux lourds et des pesticides dans des les eaux, basés sur l'inhibition enzymatique d'Arthrospira platensis

Tekaya, N. ; Saiapina, O ; Ben Ouada, H. ; Lagarde, F ; Namour, P. ; Ben Ouada, H. ; Jaffrezic Renault, N.

Type de document
Article de revue scientifique à comité de lecture
Langue
Anglais
Affiliation de l'auteur
UNIVERSITE DE LYON UMR 5280 INSTITUT DES SCIENCES ANALYTIQUES VILLEURBANNE FRA ; INSTITUTE OF MOLECULAR BIOLOGY AND GENETICS KIEV UKR ; UNIVERSITE DE MONASTIR TUN ; UNIVERSITE DE LYON UMR 5280 INSTITUT DES SCIENCES ANALYTIQUES VILLEURBANNE FRA ; IRSTEA LYON UR MALY FRA ; FACULTE DES SCIENCES UNIVERSITE DE MONASTIR TUN ; UNIVERSITE DE LYON UMR 5280 INSTITUT DES SCIENCES ANALYTIQUES VILLEURBANNE FRA
Année
2014
Résumé / Abstract
An original bi-enzymatic biosensor was designed by immobilizing Arthrospira platensis cells, called Spirulina, on gold interdigitated transducers. Phosphatase and esterase activities were inhibited, respectively, by heavy metals and by pesticides. Inhibition activities were observed with different mixtures of pesticides + heavy metals. The quantification limits for Cd2+ and Hg2+ are 10−20 M in mixture and in pure solution. The quantification limits of parathion-methyl, paraoxon-methyl and triazine are respectively 10−20 M, 10−18 M and 10−20 M in mixture and pure solutions. These results show that there is no synergistic effect between the two families of pollutants. Qualitative contamination of effluent samples and their purification after passing through a municipal wastewater treatment plant were observed by our bi-enzymatic biosensor and confirmed by classical analytical techniques for heavy metal ions.
Source
Journal of Environmental Protection, vol. 5, p. 441 - 453

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